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Luminescent cells (THP-G8 cells*) for in vitro skin sensitisation test（IL-8 Luc Assay, OECD TG 442E）　

THP-G8 cells are utilized for the in vitro skin sensitisation test (IL-8 Luc Assay), which is approved as an internationally recognized testing protocol by the Organization for Economic Co-operation and Development (OECD).

The THP-G8 cells are derived from human acute monocytic leukemia cell line (THP-1 cells) stably introducing luciferase genes, in which orange-emitting luciferase (Stable Luciferase Orange, SLO) is expressed under the control of interleukin-8 (IL-8) promoter, and red-emitting luciferase (Stable Luciferase Red, SLR) is expressed under the control of GAPDH promoter, used as an internal control reporter.

To evaluate skin sensitisation, transcriptional activation of interleukin-8 (IL-8) promoter by test chemicals, seen in human dendritic cells in skin, is simply measured by luciferase reporter assay.

* THP-G8 cells were established by Department of Dermatology, Tohoku University School of Medicine (Takahashi T. et al. Toxicol. Sci., 124, 359-369, 2011).

References

  • Takahashi T. et al., (2011) An in vitro test to screen skin sensitizers using a stable THP-1-derived IL-8 reporter cell line, THP-G8. Toxicol. Sci., 124, 359-369.
  • Kimura Y. et al., (2015) Optimization of the IL-8 Luc assay as an in vitro test for skin sensitization. Toxicol. In Vitro, 29, 1816-1830.
  • Kimura Y. et al., (2018) Profiling the immunotoxicity of chemicals based on in vitro evaluation by a combination of the Multi-ImmunoTox assay and the IL-8 Luc assay. Arch. Toxicol., 92, 2043-2054.
  • Kimura Y. et al., (2018)　The performance of an in vitro skin sensitisation test, IL-8 Luc assay (OECD442E), and the integrated approach with direct peptide reactive assay (DPRA). J. Toxicol. Sci., 43, 741-749.
  • Kimura Y. et al., (2021) The modified IL-8 Luc assay, an in vitro skin sensitisation test, can improve the false-negative judgment of lipophilic sensitizers with logKow values > 3.5. Arch. Toxicol., 95, 749-758.
  • This is a cell-only product.
  • This product is for research purposes only.
  • Protocol
  • Data sheet
  • Order form
Luminescent cells (2H4 cells*) for in vitro immunotoxicity test（IL-2 Luc Assay, OECD TG 444A）

2H4 cells are utilized for the in vitro immunotoxicity test (IL-2 Luc Assay), which is approved as an internationally recognized testing protocol by the Organization for Economic Co-operation and Development (OECD).

2H4 cells are derived from human acute T lymphoblastic cell line (Jurkat cells) stably introducing luciferase genes, in which green-emitting luciferase (Stable Luciferase Green, SLG) is expressed under the control of interleukin-2 (IL-2) promoter, orange-emitting luciferase (Stable Luciferase Orange, SLO) is expressed under the control of interferon (IFN)- γ promoter, and red-emitting luciferase (Stable Luciferase Red, SLR) is expressed under the control of GAPDH promoter, used as an internal control reporter.

To evaluate immunotoxicity, transcriptional change of IL-2 promoter of human T cells by immunotoxic chemicals is simply measured by luciferase reporter assay.

*2H4 cells were established by Tsuruga Institute of Biotechnology, TOYOBO CO., LTD., and Department of Dermatology, Tohoku University School of Medicine (Saito R. et al.(2011) Toxicol. Appl. Pharmacol., 254, 245-255).

References

  • Saito, R. et al. (2011) Nickel differentially regulates NFAT and NF-kappaB activation in T cell signaling. Toxicol. Appl. Pharmacol., 254, 245-255.
  • Kimura Y. et al., (2020) An international validation study of the IL-2 Luc assay for evaluating the potential immunotoxic effects of chemicals on T cells and a proposal for reference data for immunotoxic chemicals. Toxicol. In Vitro, 66, 104832.
  • Kimura Y. et al., (2021) Optimization of the IL-2 Luc assay for immunosuppressive drugs: a novel in vitro immunotoxicity test with high sensitivity and predictivity. Arch. Toxicol., 95, 2755-2768.
  • Kimura Y. et al., (2023) An international validation study of the interleukin-2 luciferase leukocyte toxicity test (IL-2 Luc LTT) to evaluate potential immunosuppressive chemicals and its performance after use with the interleukin-2 luciferase assay (IL-2 Luc assay). Toxicol. In Vitro, 88, 105535.
  • This is a cell-only product.
  • This product is for research purposes only.
  • Protocol
  • Data sheet
  • Order form

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